: Utilizing the program for your qPCR thermocycler, figure out at which cycle the fluorescence intensity crosses a person‐specified threshold. Making use of favourable and negative controls as benchmarks, select a threshold that clearly distinguishes amplification from non‐amplification. Skip to major content Thanks for visiting mother nature.com. That you are https://www.facebook.com/NewTechFind/posts/pfbid0YPAzGvVz72mKah68ctQg3wP1JFbs9jq9dyoRfz9AkydHS5u7wshhDSMEEcCg7NQCl